Knowing the pros and cons of each of these features on both the cytometer and analysis side we can alter how we approach our experiments to get the best possible data.
COMPENSATION IN FLOWJO MANUAL
While we wouldn’t recommend users to do manual compensation, it is occasionally valuable to make manual adjustments to the matrix since compensation isn’t always perfect and some slight over or under compensation needs to be corrected. On top of this, there is also the ability to manually create the compensation matrix on both the cytometer side and in the analysis software. This can be confusing though, why have both, and when is it right to choose one over the other.
COMPENSATION IN FLOWJO SOFTWARE
In the process of performing flow cytometry experiments you’ll notice that while we almost always calculate the compensation matrix on the cytometer using the automated compensation tool, we also have the ability to do this in our analysis software such as FlowJo.
COMPENSATION IN FLOWJO REGISTRATION
Don't miss this opportunity to learn from the very best cytometrist of the UK ! Registration and more details are HERE.Ĭompensation in DIVA vs Compensation in FlowJo and a Look into the Future Places are limited to ensure maximum interaction. The course is supported by FlowJo and FCSExpress and delegates will have access to recorded tutorials as well as the data sets used in the course. They cover some of the common applications of flow - multicolour phenotyping (including compensation), cell proliferation (DNA cell cycyle and dye dilution), and funcional assays (apoptosis and calcium flux). This course is aimed at those new to cytometry or those who want to expand their capabilities. Is there one better than the other or do they complement each other ? Find out !įinally, the Royal Microscopical Society is running an interesting Flow Cytometry Data Analysis course from March 7th to March 10th. Additionnally, keep in mind that Kevin, Francisco and I are always available for sorts and trainings on either sites !įor this month FACS Tips, we are adressing the often asked question about compensation in DIVA vs Flowjo. As a consequence, the sorting schedule starting 2050 and + has been opened since he will undoubtedly take over Daddy Danny's Legacy.ĭanny is already back at the Agora with Mariela ! So the team is ready to help you in your projects. He is the proud and happy father of Adam Friedrich Labes, born on January 16th ❤️. I would like to announce that our beloved Danny is back at the Agora. FCF UNIL February 2022 (Compensation in DIVA vs Flowjo)Īs the snow ❄️ and the health restrictions 😷 are melting away, I hope you are feeling good in this month of February !
0 kommentar(er)
